Fa yi xue za zhi, 2008; 24 (4) doi:
Affiliation: Sichuan University, China
Abstract: OBJECTIVE: To establish a fluorescent multiple amplification system of 16S rRNA and Cytb genes located in mitochondrial DNA for species identification.
METHODS: A pair of primers of 16S rRNA gene and Cytb gene of the mitochondrial DNA was designed with the software Primer 5.0 to construct a multiple amplification system. The amplified products from human and five species of animals, including cattle, pig, dog, chicken and grass carp were analyzed by 310 Genetic Analyzer.
RESULTS: The amplified products of these samples showed two peaks. The common one was 358bp and the specific one different in unique species was between 231bp and 256bp.
CONCLUSION: The multiplex amplification system can exactly distinguish the species of human from five common animals.
The top research papers for the subject are placed on the map. Studies form clusters based on semantic relation.
Size of the point represents relevance of the paper.
You can pan and zoom the graph using mouse and mouse wheel.
Right click on the paper to:
Left click on keyword to add it to search.
Sign up to create your own map!
Working in Life Sciences?
Understand your Field of Research.
Explore the Life Sciences from Big Data point of view.